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5 March 2021 In vivo multimodal photoacoustic microscopy and optical coherence tomography longitudinal tracking of progenitor cells labeled with functionalized chain-like gold nanoparticle clusters after ocular subretinal transplantation
Van Phuc Nguyen, Tianye Zhu, Wen Fan, Wei Qian, Yanxiu Li, Wei Zhang, Jessica Henry, Bing Liu, Xueding Wang, Yannis M. Paulus
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Proceedings Volume 11642, Photons Plus Ultrasound: Imaging and Sensing 2021; 116422R (2021) https://doi.org/10.1117/12.2577647
Event: SPIE BiOS, 2021, Online Only
Abstract
Regenerative therapies such as stem cell therapies are an area of active investigation that will likely play an important role in the future to improve vision loss from retinal diseases including macular degeneration and retinitis pigmentosa. It is important to visualize these cells after administration to determine their fate and effect. In this study, an advanced non-invasive photoacoustic microscopy (PAM) and optical coherence tomography (OCT) imaging system was developed to monitor cells in vivo. To boost the sensitivity of PAM and OCT, novel ultrapure functionalized chain-like gold nanoparticle (CGNP) clusters were synthesized and cultured into a precursor human retinal pigment epithelial cell line with differentiated properties (ARPE-19) cells. The fabricated CGNP clusters have redshifted plasmonic peak absorption from 520nm to 650nm, resulting in reduced background signal from hemoglobin. The position of cells following subretinal injection into the rabbit retina having laser injury is selectively tracked longitudinally in vivo using integrated photoacoustic microscopy (PAM) and OCT over 3 months in 3 rabbits. PAM images obtained at two different optical wavelengths of 578 nm and 650 nm were overlaid on the same image plane and on the OCT image allowed to distinguish transplanted cells from the adjacent native choroidal vessels and track the migration of the cells over time. Quantification of PAM and OCT signals illustrated that the PAM signal increased by 30-fold, and OCT signal increased by 180 %. Histological analysis confirmed that ARPE-19 cells migrated to the injured sites and correlated with the location noted on the PAM/OCT imaging. This work provides a comprehensive imaging and nanoparticle system that could be used for labeling and tracking of cell-based regenerative therapies.
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Van Phuc Nguyen, Tianye Zhu, Wen Fan, Wei Qian, Yanxiu Li, Wei Zhang, Jessica Henry, Bing Liu, Xueding Wang, and Yannis M. Paulus "In vivo multimodal photoacoustic microscopy and optical coherence tomography longitudinal tracking of progenitor cells labeled with functionalized chain-like gold nanoparticle clusters after ocular subretinal transplantation", Proc. SPIE 11642, Photons Plus Ultrasound: Imaging and Sensing 2021, 116422R (5 March 2021); https://doi.org/10.1117/12.2577647
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KEYWORDS
Optical coherence tomography
In vivo imaging
Nanoparticles
Photoacoustic microscopy
Gold
Optical tracking
Transplantation
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