Presentation
5 March 2021 Advancing impulsive stimulated Brillouin microscopy using a femtosecond laser for biomechanics
Author Affiliations +
Abstract
In biomedicine, the mechanical properties of cells and tissues are increasingly recognized as a diagnostic tool. Impulsive stimulated Brillouin scattering (ISBS) is promising to overcome the long measurement durations of the established (spontaneous) Brillouin microscopy. ISBS microscopy offers fast three-dimensional non-contact measurements on in vivo samples. In ISBS microscopy, a standing acoustic wave is excited by an interference fringe pattern generated by an ultra-short pulsed laser. The oscillation frequency and therefore local mechanical properties can be retrieved using a cw probe laser. We will present the possibilities, limits and trade-offs for ISBS microscopy in biomedical applications.
Conference Presentation
© (2021) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Benedikt Krug, Nektarios Koukourakis, and Jürgen W. Czarske "Advancing impulsive stimulated Brillouin microscopy using a femtosecond laser for biomechanics", Proc. SPIE 11645, Optical Elastography and Tissue Biomechanics VIII, 116450R (5 March 2021); https://doi.org/10.1117/12.2577497
Advertisement
Advertisement
KEYWORDS
Microscopy

Femtosecond phenomena

Continuous wave operation

Signal to noise ratio

Spatial resolution

Photodiodes

Pulsed laser operation

Back to Top