An overview of continuous and discrete phasor analysis of binned or time-gated periodic decays

X. Michalet

doi:10.1117/12.2577747

5 March 2021 An overview of continuous and discrete phasor analysis of binned or time-gated periodic decays

X. Michalet

Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116480E (2021) https://doi.org/10.1117/12.2577747
Event: SPIE BiOS, 2021, Online Only

Abstract

Time-resolved analysis of periodically excited luminescence decays by the phasor method in the presence of time-gating or binning is revisited. Analytical expressions for discrete configurations of square gates are derived and the locus of the phasors of such modified periodic single-exponential decays is compared to the canonical universal semicircle. The effects of IRF offset, decay truncation and gate shape are also discussed. Finally, modified expressions for the phase and modulus lifetimes are provided for some simple cases. A discussion of a modified phasor calibration approach is presented.

Conference Presentation

Citation Download Citation

X. Michalet "An overview of continuous and discrete phasor analysis of binned or time-gated periodic decays", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116480E (5 March 2021); https://doi.org/10.1117/12.2577747

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available