Computational Hybrid Imaging: Measure Fluorescence and Refractive Index Together

Yi Xue; Laura Waller

doi:10.1117/12.2579940

5 March 2021 Computational Hybrid Imaging: Measure Fluorescence and Refractive Index Together

Yi Xue, Laura Waller

Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 116490W (2021) https://doi.org/10.1117/12.2579940
Event: SPIE BiOS, 2021, Online Only

Abstract

Optical fluorescence microscopy has been widely used to image neurons in the brain. When imaging deep inside of the brain, image signal-to-noise ratio (SNR) severely degrades by scattering, caused by the heterogeneity of different structures. Knowing the refractive index (RI) of the structures can reduce scattering effects and improve image SNR. Currently, most of RI imaging is based on coherent or partial coherent measurements of the transmitted scattered light field, whereas fluorescence microscopy for brain imaging only records incoherent fluorescence signal propagating back to the objective lens in epi-mode. Here, we propose a model to calculate the RI of the 3D scattering tissue only from fluorescence images in reflective geometry. Our method not only successfully reconstructed the RI of a 3D phantom, but also acquired fluorescence images and the unlabeled structures in the same volume simultaneously.

Conference Presentation

Citation Download Citation

Yi Xue and Laura Waller "Computational Hybrid Imaging: Measure Fluorescence and Refractive Index Together", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 116490W (5 March 2021); https://doi.org/10.1117/12.2579940

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available