DOF adjustment and 3D free viewpoint viewing of Z stack data of fluorescent microscope using image processing

Tsuneyuki Hagiwara

doi:10.1117/12.2577412

5 March 2021 DOF adjustment and 3D free viewpoint viewing of Z stack data of fluorescent microscope using image processing

Tsuneyuki Hagiwara

Author Affiliations +

Proceedings Volume 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII; 1164915 (2021) https://doi.org/10.1117/12.2577412
Event: SPIE BiOS, 2021, Online Only

Abstract

In recent years, confocal fluorescence microscopes have become widespread and many Z-stack data have been collected. However, it is the current situation that there is hardly a method for three-dimensional observation of huge amounts of data with sufficient resolution and without loss of information. In light of this situation, we have developed an image processing algorithm, referred to as arbitrary blur algorithm (ABA), which enables post-adjustment of DOF and 3D image observation from a free viewpoint, with high resolution and without loss of information. In this report, we first explain the principle of image processing that enables depth of field (DOF) adjustment and 3D image observation from a free viewpoint using Z-stack data. Next, the results of the simulation are introduced. In the simulation, we show the comparison result of the point image by MIP (maximum intensity projection) method and the point image of the proposed method and clarify the superiority of the proposed method. Next, we introduce the results of the experimental verification. For experimental verification, we show the results using wide field fluorescence microscope Z-stack data and confocal fluorescence microscope Z-stack data. As confocal data, observation examples of mouse hippocampal neuron synapses are shown in comparison with the results displayed by existing software.

Conference Presentation

Citation Download Citation

Tsuneyuki Hagiwara "DOF adjustment and 3D free viewpoint viewing of Z stack data of fluorescent microscope using image processing", Proc. SPIE 11649, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII, 1164915 (5 March 2021); https://doi.org/10.1117/12.2577412

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available