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5 March 2021 Time-domain single photon-excited autofluorescence lifetime is sensitive to activation state of T cells
Kayvan Samimi, Emmanuel Contreras Guzman, Steven M. Trier, Dan Pham, Tongcheng Qian, Melissa C. Skala
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Proceedings Volume 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021; 116550L (2021) https://doi.org/10.1117/12.2577765
Event: SPIE BiOS, 2021, Online Only
Abstract
A single photon-excited confocal whole-cell autofluorescence lifetime system is described that uses fast field-programmable gate array (FPGA)-based time tagging electronics to implement time-correlated single photon counting (TCSPC) with simultaneous near-IR brightfield imaging. This system resolves variations in the fluorescence decay of the metabolic coenzyme NAD(P)H that provides high accuracy and precision for classifying activated and quiescent primary human T cells (ROC AUC = 0.98). This performance is similar to that obtained using commercial two-photon fluorescence lifetime imaging microscopy (FLIM). The small footprint, low cost, and high acquisition speed make this system attractive for monitoring cell biomanufacturing.
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Kayvan Samimi, Emmanuel Contreras Guzman, Steven M. Trier, Dan Pham, Tongcheng Qian, and Melissa C. Skala "Time-domain single photon-excited autofluorescence lifetime is sensitive to activation state of T cells", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116550L (5 March 2021); https://doi.org/10.1117/12.2577765
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KEYWORDS
Fluorescence lifetime imaging
Flow cytometry
Imaging systems
Microscopy
Nondestructive evaluation
Proteins
Resistance
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