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5 March 2021 Background noise suppressed high-throughput mid-infrared photothermal microscopy via pupil engineering
Haonan Zong, Celalettin Yurdakul, Yeran Bai, Meng Zhang, Selim Ünlü, Ji-Xin Cheng
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Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 116560K (2021) https://doi.org/10.1117/12.2578733
Event: SPIE BiOS, 2021, Online Only
Abstract
Mid-infrared photothermal microscopy (MIP) has been a promising chemical imaging technique for functionality characterization of biological and pharmaceutical specimens owing to its enhanced resolution and high-specificity. Recently developed wide-field MIP modalities drastically improved the imaging speed and thus enabled high-throughput imaging. However, the sensitivity of the wide-field MIP technique has been limited by shot-noise of background photons. Here, we demonstrate a dark-field MIP modality to allow 4-fold signal-to-noise ratio improvement. Our technique is based on selectively blocking the reflected light. Simulation and experimental results are both provided, and they are consistent with each other.
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Haonan Zong, Celalettin Yurdakul, Yeran Bai, Meng Zhang, Selim Ünlü, and Ji-Xin Cheng "Background noise suppressed high-throughput mid-infrared photothermal microscopy via pupil engineering", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116560K (5 March 2021); https://doi.org/10.1117/12.2578733
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KEYWORDS
Microscopy
Mid-IR
Particles
Image resolution
Imaging spectroscopy
Light scattering
Photons
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