Raman microscopy with dual-wavelength excitation for live cell imaging to detect resonance and non-resonance Raman scattering

Yasunori Nawa; Yasuaki Kumamoto; Menglu Li; Satoshi Fujita; Katsumasa Fujita

doi:10.1117/12.2579188

5 March 2021 Raman microscopy with dual-wavelength excitation for live cell imaging to detect resonance and non-resonance Raman scattering

Yasunori Nawa, Yasuaki Kumamoto, Menglu Li, Satoshi Fujita, Katsumasa Fujita

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Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 116560Y (2021) https://doi.org/10.1117/12.2579188
Event: SPIE BiOS, 2021, Online Only

Abstract

Resonance Raman scattering is useful for improving a signal-to-noise ratio and a data acquisition speed in Raman imaging. However, the detection of non-resonance Raman scattering is often hindered by resonance signals and fluorescent background. To aid this dilemma in using resonance Raman scattering, we have developed a confocal Raman microscope with dual-wavelength excitation. Living HeLa cells were measured simultaneously at two different excitation wavelengths. At 532 nm excitation, cytochromes were detected by the resonance effect. At 660 nm excitation, non-resonance signals from proteins and lipids were obtained without any clear influence from cytochromes and fluorescent background.

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Yasunori Nawa, Yasuaki Kumamoto, Menglu Li, Satoshi Fujita, and Katsumasa Fujita "Raman microscopy with dual-wavelength excitation for live cell imaging to detect resonance and non-resonance Raman scattering", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116560Y (5 March 2021); https://doi.org/10.1117/12.2579188

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KEYWORDS

Raman spectroscopy

Raman scattering

Live cell imaging

Microscopy

Molecules

Microscopes

Molecular spectroscopy

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