Raman chemical imaging of polymer-mediated gene delivery

David Punihaole; Craig Van Bruggen; Renee Frontiera; Theresa Reineke

doi:10.1117/12.2577317

5 March 2021 Raman chemical imaging of polymer-mediated gene delivery

David Punihaole, Craig Van Bruggen, Renee Frontiera, Theresa Reineke

Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 1165611 (2021) https://doi.org/10.1117/12.2577317
Event: SPIE BiOS, 2021, Online Only

Abstract

Polymer-based vehicles that controllably deliver therapeutic nucleic acids to cells show great potential to develop safe and effective gene therapies. Realizing this potential, however, is limited by the lack of understanding of how polymeric vehicles unpackage and release their cargo in the cell. We address this problem by utilizing a novel quinine-containing polymeric vehicle that shows exceptional gene delivery activity. A key aspect of this platform is that the quinine serves as a reporter for DNA binding, which allows us to track cargo release inside the cell using chemical imaging. We find that proteins dominate the unpackaging of DNA encapsulated by these quinine polymers inside cells. This trackable delivery system should be broadly applicable to study gene delivery mechanisms, as well as be used for clinical therapy applications.

Conference Presentation

Citation Download Citation

David Punihaole, Craig Van Bruggen, Renee Frontiera, and Theresa Reineke "Raman chemical imaging of polymer-mediated gene delivery", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 1165611 (5 March 2021); https://doi.org/10.1117/12.2577317

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