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5 March 2021 Quantitative assessment of fluorescence dyes and staining protocols for virtual hematoxylin and eosin (H&E) imaging using ex vivo microscopy
Ivan Bozic, Madeline R. Behr, Jonathon Q. Brown
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Proceedings Volume 11660, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XIII; 1166007 (2021) https://doi.org/10.1117/12.2578455
Event: SPIE BiOS, 2021, Online Only
Abstract
Staining procedures in virtual H&E ex vivo microscopy is non-standardized, and image quality can be tuned by systematic parameter optimization. We are using structured illumination microscopy and image processing for an objective assessment of staining procedure. Bovine muscle tiles of 2x2 cm are used as experimental tissue. In these experiments we differ staining procedure by varying stain and rinse solvent, dye concentration, and immersion time. Quantitative measures of staining quality were compared. Our findings confirm the profound impact of the confluence of multiple factors in staining quality, and suggest general strategies for successful protocol development.
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Ivan Bozic, Madeline R. Behr, and Jonathon Q. Brown "Quantitative assessment of fluorescence dyes and staining protocols for virtual hematoxylin and eosin (H&E) imaging using ex vivo microscopy", Proc. SPIE 11660, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XIII, 1166007 (5 March 2021); https://doi.org/10.1117/12.2578455
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KEYWORDS
Microscopy
Multiphoton microscopy
Luminescence
Image quality
Multiphoton fluorescence microscopy
Prostate cancer
Signal to noise ratio
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