Wavelength coded volume holographic gratings based dual wavelength fluorescence imaging system

Yu-Hsin Chia; Yi-You Huang; Yuan Luo

doi:10.1117/12.2585463

5 March 2021 Wavelength coded volume holographic gratings based dual wavelength fluorescence imaging system

Yu-Hsin Chia, Yi-You Huang, Yuan Luo

Proceedings Volume 11709, Ultra-High-Definition Imaging Systems IV; 117090U (2021) https://doi.org/10.1117/12.2585463
Event: SPIE OPTO, 2021, Online Only

Conference Poster

Abstract

We present a wavelength coded volume holographic gratings (WC-VHGs) based dual wavelength fluorescence imaging system to simultaneously obtain two color fluorescence images of biological samples in one shot. The system utilizes wavelength coded recording technique based on Bragg degeneracy to generate the PQ-PMMA volume holographic gratings, which has high selectivity in both wavelength and angle. In order to improve the optical sectioning ability of the proposed system, we use HiLo image processing to suppress the out of focus signal. Experimental results demonstrate the imaging ability of the proposed system to observe multi-wavelength fluorescence images and HiLo images.

Conference Presentation

Citation Download Citation

Yu-Hsin Chia, Yi-You Huang, and Yuan Luo "Wavelength coded volume holographic gratings based dual wavelength fluorescence imaging system", Proc. SPIE 11709, Ultra-High-Definition Imaging Systems IV, 117090U (5 March 2021); https://doi.org/10.1117/12.2585463

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