Imaging membrane interfaces and secondary protein conformation in cell cultures with vibrational infrared photothermal and phase signal (VIPPS) microscopy

Panagis D. Samolis; Daniel Langley; Breanna M. O'Reilly; Zay Oo; Geva Hilzenrat; Shyamsunder Erramilli; Allyson E. Sgro; Sally McArthur; Michelle Y. Sander

doi:10.1117/12.2595736

1 August 2021 Imaging membrane interfaces and secondary protein conformation in cell cultures with vibrational infrared photothermal and phase signal (VIPPS) microscopy

Panagis D. Samolis, Daniel Langley, Breanna M. O'Reilly, Zay Oo, Geva Hilzenrat, Shyamsunder Erramilli, Allyson E. Sgro, Sally McArthur, Michelle Y. Sander

Author Affiliations +

Proceedings Volume 11825, Ultrafast Nonlinear Imaging and Spectroscopy IX; 118250J (2021) https://doi.org/10.1117/12.2595736
Event: SPIE Optical Engineering + Applications, 2021, San Diego, California, United States

Abstract

Mid-infrared photothermal microscopy has demonstrated unique capabilities in the field of chemical imaging including sub-diffraction limited resolution and sub cellular imaging. Vibrational Infrared Photothermal and Phase Signal (VIPPS) introduces an additional contrast mechanism based on different thermal properties. This enables high contrast imaging of features with overlapping absorption profiles but different thermal diffusion characteristics. Our approach paves the way for high contrast sub-diffraction limited imaging of secondary protein conformations in fibroblast cells grown in a protein rich collagen matrix at the subcellular level.

Conference Presentation

Citation Download Citation

Panagis D. Samolis, Daniel Langley, Breanna M. O'Reilly, Zay Oo, Geva Hilzenrat, Shyamsunder Erramilli, Allyson E. Sgro, Sally McArthur, and Michelle Y. Sander "Imaging membrane interfaces and secondary protein conformation in cell cultures with vibrational infrared photothermal and phase signal (VIPPS) microscopy", Proc. SPIE 11825, Ultrafast Nonlinear Imaging and Spectroscopy IX, 118250J (1 August 2021); https://doi.org/10.1117/12.2595736

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