Histological and cytological imaging using Fourier ptychographic microscopy

Christopher Bendkowski; Bin Fu; Petru Manescu; Rémy Claveau; Delmiro Fernandez-Reyes; Michael Shaw

doi:10.1117/12.2604455

6 October 2021 Histological and cytological imaging using Fourier ptychographic microscopy

Christopher Bendkowski, Bin Fu, Petru Manescu, Rémy Claveau, Delmiro Fernandez-Reyes, Michael Shaw

Proceedings Volume 11879, Frontiers in Biophotonics and Imaging; 1187902 (2021) https://doi.org/10.1117/12.2604455
Event: SPIE Photonex, 2021, Glasgow, United Kingdom

Abstract

Structural imaging using light microscopy is a cornerstone of histology and cytology. However, the utility of the optical microscope for diagnostic imaging is limited by the fundamental tradeoff between the field of view and spatial resolution and a reliance on exogenous dyes to generate sufficient image contrast. Fourier Ptychographic Microscopy (FPM) is a complex imaging modality with the potential to overcome these limitations by recovering high-resolution images of sample amplitude and phase from a set of low-resolution raw images captured under inclined illumination. In this article we explore the application of FPM to clinical imaging using a simple, low-cost FPM system and simulated and experimental data to explore the influence of both image acquisition parameters and hardware configuration on image quality and imaging throughput. The practical performance of the method is investigated by imaging peripheral blood films and histological tissue sections. We find that, at the cost of increased computational complexity, FPM increases the information capture capacity of the optical microscope significantly, allowing label-free examination and quantification of features such as tissue and cell morphology over large sample areas.

Conference Presentation

Citation Download Citation

Christopher Bendkowski, Bin Fu, Petru Manescu, Rémy Claveau, Delmiro Fernandez-Reyes, and Michael Shaw "Histological and cytological imaging using Fourier ptychographic microscopy", Proc. SPIE 11879, Frontiers in Biophotonics and Imaging, 1187902 (6 October 2021); https://doi.org/10.1117/12.2604455

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