Physiological modulation of a biological cell by using direct electron beam exposure

Asahi Tanaka; Wataru Inami; Yoshimasa Kawata

doi:10.1117/12.2615411

27 October 2021 Physiological modulation of a biological cell by using direct electron beam exposure

Asahi Tanaka, Wataru Inami, Yoshimasa Kawata

Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 1192508 (2021) https://doi.org/10.1117/12.2615411
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

We have developed a stimulation method for a living cell by using electron beam exposure. Liquid water is a major chemical component of a cell and is expected to be decomposed into reactive oxygen species (ROS) upon the beam exposure, which is known as a cellular signaling molecule and modulates various cellular functions. The beam target cell is stained with a fluorescence probe, CM-H2DCFDA which is sensitive to intracellular ROS and emits bright fluorescence once it reacts with ROS. By comparing the fluorescence intensity of the target cell before and after the beam exposure, it is implied that intracellular ROS is generated by electron beam irradiation. Therefore, this method can modulate cellular physiology through artificial generation of ROS, which is applied for future biological researches.

Citation Download Citation

Asahi Tanaka, Wataru Inami, and Yoshimasa Kawata "Physiological modulation of a biological cell by using direct electron beam exposure", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 1192508 (27 October 2021); https://doi.org/10.1117/12.2615411

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