Application of infrared laser to living cells for manipulation of gene expression, and in vivo temperature measurement method

Takumi Tomoi; Joe Sakamoto; Suguru Ohe; Yosuke Tamada; Yasuhiro Kamei

doi:10.1117/12.2615414

27 October 2021 Application of infrared laser to living cells for manipulation of gene expression, and in vivo temperature measurement method

Takumi Tomoi, Joe Sakamoto, Suguru Ohe, Yosuke Tamada, Yasuhiro Kamei

Author Affiliations +

Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 119250A (2021) https://doi.org/10.1117/12.2615414
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

Combination of infrared laser heating through a microscope optics and heat shock response of cells achieves single-cell gene induction in vivo. We will introduce a live-cell manipulation technique and show representative data in living animals and plants. Further, we will discuss a local heating property in vivo through a temperature imaging technique using a fluorescent thermometer.

Citation Download Citation

Takumi Tomoi, Joe Sakamoto, Suguru Ohe, Yosuke Tamada, and Yasuhiro Kamei "Application of infrared laser to living cells for manipulation of gene expression, and in vivo temperature measurement method", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 119250A (27 October 2021); https://doi.org/10.1117/12.2615414

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