3D imaging through Fourier lightfield microscopy

Genaro Saavedra; Emilio Sanchez-Ortiga; Hui Yun; Gabriele Scrofani; Manuel Martinez-Corral

doi:10.1117/12.2615665

27 October 2021 3D imaging through Fourier lightfield microscopy

Genaro Saavedra, Emilio Sanchez-Ortiga, Hui Yun, Gabriele Scrofani, Manuel Martinez-Corral

Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 119250M (2021) https://doi.org/10.1117/12.2615665
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

Lightfield (also known as integral or plenoptic) microscopy has been introduced successfully in 3D bioimaging. The strongest point of this technique when compared with conventional 3D microscopy arquitectures is its capability of capturing the 3D information of the sample in a single-shot manner. Despite this advantage, lightfield microscopy currently faces some challenges, like improving the resolution and depth of field of the reconstructed specimens or the development and optimization of specially-adapted reconstruction algorithms. In this contribution we review a new paradigm, namely, the Fourier lightfield microscope (FLMic), that improves the capabilities of the technique and we present recent advances and applications of this new architecture. As an example, we describe a protocol that takes profit from FLMic concept for providing, in a single-shot, 3D dark-field images of volumetric transparent samples.

Citation Download Citation

Genaro Saavedra, Emilio Sanchez-Ortiga, Hui Yun, Gabriele Scrofani, and Manuel Martinez-Corral "3D imaging through Fourier lightfield microscopy", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 119250M (27 October 2021); https://doi.org/10.1117/12.2615665

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