Imaging mechanical properties of cancer cells during metastasis with Brillouin microspectroscopy

Vsevolod Cheburkanov; Kavya Pendyala; Maria Parlani; Tanmay Lele; Peter Friedl; Vladislav Yakovlev

doi:10.1117/12.2610539

4 March 2022 Imaging mechanical properties of cancer cells during metastasis with Brillouin microspectroscopy

Vsevolod Cheburkanov, Kavya Pendyala, Maria Parlani, Tanmay Lele, Peter Friedl, Vladislav Yakovlev

Proceedings Volume 11944, Multiscale Imaging and Spectroscopy III; 119440C (2022) https://doi.org/10.1117/12.2610539
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Cancer remains among the leading causes of death in the United States. Early detection, classification and understanding of malignant cell proliferation and metastasis mechanisms are crucial for effective treatment. Current malignant cell studies largely rely on either invasive imaging techniques or invasive research protocols that hinder both speed and accuracy of cancer research. Here we are reporting successful imaging of cancer metastasis processes on a cellular level using Brillouin microspectroscopic imaging. In this research we are specifically presenting results of a non-invasive interrogation of elastic properties of 4T1 murine fibroblast cells in a spheroid model acquired with our custom-built confocal Brillouin microspectrometer. Spatial map of elastic properties was recorded for both interior and exterior regions of the 4T1 cell spheroid. We observed lower stiffness of cancer cells compared to cells from internal regions. In addition we observed the difference in stiffness values between cells exposed to challenging and normal environmental conditions. Our findings correlate well with prior published data, acquired with conventional biomechanical assessment techniques.

Conference Presentation

Citation Download Citation

Vsevolod Cheburkanov, Kavya Pendyala, Maria Parlani, Tanmay Lele, Peter Friedl, and Vladislav Yakovlev "Imaging mechanical properties of cancer cells during metastasis with Brillouin microspectroscopy", Proc. SPIE 11944, Multiscale Imaging and Spectroscopy III, 119440C (4 March 2022); https://doi.org/10.1117/12.2610539

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available