A compact open-top light-sheet microscope for Optofluidic imaging

Jeonghwan Son; Biagio Mandracchia; Shu Jia

doi:10.1117/12.2607324

2 March 2022 A compact open-top light-sheet microscope for Optofluidic imaging

Jeonghwan Son, Biagio Mandracchia, Shu Jia

Proceedings Volume 11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX; 1196608 (2022) https://doi.org/10.1117/12.2607324
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

The fluorescence Optofluidic imaging have recently transformed conventional optical investigations of biological systems by exploiting the synergic combination of recent microscopy and integrated chips technologies. The implementation of light-sheet microscopy on Optofluidic imaging has further pushed the systemic capabilities towards fast and 3D-volumetric imaging. Here, we propose a new compact open-top light-sheet microscope to extend the study of 3D-fluorescence Optofluidic imaging. The proposed portable architecture of the system and customized reconstruction algorithms enable providing high throughput and volumetric reconstruction capability with an isometric single-cell resolution. The experimental results demonstrate the applicability of open-top light-sheet configuration to single-cell imaging in a flow. We anticipate the system to offer promising applications in clinical settings where the portability and 3D-volumetric analysis are required for fast diagnostics.

Conference Presentation

Citation Download Citation

Jeonghwan Son, Biagio Mandracchia, and Shu Jia "A compact open-top light-sheet microscope for Optofluidic imaging", Proc. SPIE 11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX, 1196608 (2 March 2022); https://doi.org/10.1117/12.2607324

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