High-resolution volumetric imaging using hybrid image of Fourier light-field microscopy and wide-field microscopy

Wenhao Liu; Shu Jia

doi:10.1117/12.2610474

2 March 2022 High-resolution volumetric imaging using hybrid image of Fourier light-field microscopy and wide-field microscopy

Wenhao Liu, Shu Jia

Author Affiliations +

Proceedings Volume 11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX; 1196609 (2022) https://doi.org/10.1117/12.2610474
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We have developed wFLFM to further enhance the resolution by taking the advantage of both wide-field image and FLFM. We proposed the principle of wFLFM and verified the approach both numerically using simulated rings, and experimentally using phantom samples on the wFLFM system we constructed as a prototype. The principle of wFLFM requires only additional wild-field image at the aperture plane, making it readily compatible with various extended depth of focus wide-field modules, and the validating results showed a 2-3× enhancement in lateral resolution without cost in axial information, thereby advancing wFLFM as a promising high-resolution 3D imaging method.

Conference Presentation

Citation Download Citation

Wenhao Liu and Shu Jia "High-resolution volumetric imaging using hybrid image of Fourier light-field microscopy and wide-field microscopy", Proc. SPIE 11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX, 1196609 (2 March 2022); https://doi.org/10.1117/12.2610474

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