Single-exposure 3D label-free microscopy based on color-multiplexed intensity diffraction tomography

Ning Zhou; Jiaji Li; Runnan Zhang; Zhidong Bai; Qian Chen; Chao Zuo

doi:10.1117/12.2606521

1 November 2021 Single-exposure 3D label-free microscopy based on color-multiplexed intensity diffraction tomography

Ning Zhou, Jiaji Li, Runnan Zhang, Zhidong Bai, Qian Chen, Chao Zuo

Proceedings Volume 12057, Twelfth International Conference on Information Optics and Photonics; 120573E (2021) https://doi.org/10.1117/12.2606521
Event: Twelfth International Conference on Information Optics and Photonics, 2021, Xi'an, China

Abstract

We present a 3D label-free refractive index (RI) imaging technique based on single-exposure intensity diffraction tomography (sIDT) utilizing a color-multiplexed illumination scheme. In our method, the chromatic LEDs corresponding R/G/B channels in an annular programmable array provide oblique illumination geometry which matches the numerical aperture of the objective precisely to maximize the spectrum coverage. A color intensity image encoding the scattering field of the specimen from different directions is captured, and monochromatic intensity images with respect to three color channels were separated and then used to recover the 3D RI distribution of the object following the process of IDT. In addition, the axial chromatic dispersion of focal lengths at different wavelengths introduced by the chromatic aberration of the objective lens and the spatial position misalignment of the ring LED source in the imaging system’s transfer functions modeling are both corrected to significantly reduce the artifacts in slice-based deconvolution procedure for the reconstruction of 3D RI distribution. Experimental results on MCF-7, Spirulina algae, and live C. elegans samples demonstrate the solid performance of the sIDT method in label-free, high-throughput, and real-time (∼ 24 fps) 3D volumetric biological imaging applications.

Citation Download Citation

Ning Zhou, Jiaji Li, Runnan Zhang, Zhidong Bai, Qian Chen, and Chao Zuo "Single-exposure 3D label-free microscopy based on color-multiplexed intensity diffraction tomography", Proc. SPIE 12057, Twelfth International Conference on Information Optics and Photonics, 120573E (1 November 2021); https://doi.org/10.1117/12.2606521

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available