Confocal microscope with enhanced depth discrimination

Roland V. Shack; Peter H. Bartels; Donald W. Hillman; M. Sabb; Richard L. Shoemaker

doi:10.1117/12.17804

1 July 1990 Confocal microscope with enhanced depth discrimination

Roland V. Shack, Peter H. Bartels, Donald W. Hillman, M. Sabb, Richard L. Shoemaker

Author Affiliations +

Proceedings Volume 1206, New Technologies in Cytometry and Molecular Biology; (1990) https://doi.org/10.1117/12.17804
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

Confocal microscopy, in comparison to conventional microscopy, offers a narrower point-spread function, the rendition of phase structures in contrast, and high axial resolution. Of these, the capabilities of optical sectioning and the three-dimensional imaging of cells and tissues have attracted the most interest. The literature offers a number of studies exploring the factors affecting depth of field in confocal microscopy. In this article, the three-dimensional representation of fluorescence imagery is examined and the design of a laser scanning confocal fluorescence microscope with enhanced depth discrimination is described.

Citation Download Citation

Roland V. Shack, Peter H. Bartels, Donald W. Hillman, M. Sabb, and Richard L. Shoemaker "Confocal microscope with enhanced depth discrimination", Proc. SPIE 1206, New Technologies in Cytometry and Molecular Biology, (1 July 1990); https://doi.org/10.1117/12.17804

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