Effects of some model approximations in the reconstructions of digital in-line holograms: simulations, experiments on calibrated objects and model refinement assessment

Thomas Olivier; Dylan Brault; Sachin Joshi; Thomas Brard; Alexey Brodoline; Loïc Méès; Corinne Fournier

doi:10.1117/12.2621668

20 May 2022 Effects of some model approximations in the reconstructions of digital in-line holograms: simulations, experiments on calibrated objects and model refinement assessment

Thomas Olivier, Dylan Brault, Sachin Joshi, Thomas Brard, Alexey Brodoline, Loïc Méès, Corinne Fournier

Author Affiliations +

Proceedings Volume 12136, Unconventional Optical Imaging III; 1213603 (2022) https://doi.org/10.1117/12.2621668
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

The in-line configuration of digital holographic microscopy is the simplest to set up, but it requires numerical reconstructions, in order to retrieve the phase of the wave diffracted by the sample. These reconstructions are based on an image formation model, but the effect of the microscopy system is often neglected. Yet, some parameters, like a wrong magnification, the partial coherence of the illumination, or optical aberrations may lead to bias in the reconstruction. In the framework of inverse problems approaches, we analysed and studied the effects of some of these parameters using simulations and experiments on calibrated spherical objects and a rigorous model (Lorenz-Mie), in order to evaluate the relevance and requirements of model refinements.

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Thomas Olivier, Dylan Brault, Sachin Joshi, Thomas Brard, Alexey Brodoline, Loïc Méès, and Corinne Fournier "Effects of some model approximations in the reconstructions of digital in-line holograms: simulations, experiments on calibrated objects and model refinement assessment", Proc. SPIE 12136, Unconventional Optical Imaging III, 1213603 (20 May 2022); https://doi.org/10.1117/12.2621668

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