Armin J. M. Lenz,1 Fernando Soldevila,2,3 Alberto Ghezzi,4,5 Andrea Farinahttps://orcid.org/0000-0002-3180-6328,6 Cosimo D'Andrea,4,7 Enrique Tajahuerce1
1Univ. Jaume I (Spain) 2Lab. Kastler Brossel, CNRS (France) 3Sorbonne Univ. (France) 4Politecnico di Milano (Italy) 5Consiglio Nazionale delle Ricerche (Italy) 6CNR-Istituto di Fotonica e Nanotecnologie (Italy) 7Istituto Italiano di Tecnologia (Italy)
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We present a technique for multispectral fluorescence lifetime imaging with high spatial resolution by combining both single-pixel and data fusion imaging techniques. The system relies on the combined use of three different sensors: two SP cameras capturing multispectral and time-resolved information, and a conventional 2D array detector capturing high spatial resolution images The resultant giga-voxel 4D hypercube is acquired in a fast manner measuring only 0.03% of the dataset. The fusion procedure is done by solving a regularization problem which is efficiently solved via gradient descent. The system can be used to identify fluorophore species.
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Armin J. M. Lenz, Fernando Soldevila, Alberto Ghezzi, Andrea Farina, Cosimo D'Andrea, Enrique Tajahuerce, "Giga-voxel multispectral time-resolved imaging with single-pixel detection and data fusion," Proc. SPIE 12136, Unconventional Optical Imaging III, 121360D (20 May 2022); https://doi.org/10.1117/12.2621587