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7 December 2022 A 32 mcps single-channel system for high-speed and low distortion microscopy: on-field measurements and future evolution
Serena Farina, Ivan Labanca, Giulia Acconcia, Ivan Rech, Alberto Ghezzi, Andrea Farina, Cosimo D'Andrea
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Proceedings Volume 12274, Emerging Imaging and Sensing Technologies for Security and Defence VII; 122740D (2022) https://doi.org/10.1117/12.2636024
Event: SPIE Security + Defence, 2022, Berlin, Germany
Abstract
Time-Correlated Single Photon Counting (TCSPC) represents a fundamental tool for the investigation of biological light signals. Unfortunately, due to pile-up distortion the photon acquisition rate must be kept below few percents of the laser rate, thus increasing the acquisition time. Recently, we proposed a single-channel TCSPC system allowing us to overcome pile-up by matching the detector dead time to the laser period. In this work, we perform on-field fluorescence measurements with this system showing that an acquisition speed of 32 Mcps can be reached without significant distortion. Thanks to the promising results, we are now developing a multi-channel module based on the same acquisition technique.
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Serena Farina, Ivan Labanca, Giulia Acconcia, Ivan Rech, Alberto Ghezzi, Andrea Farina, and Cosimo D'Andrea "A 32 mcps single-channel system for high-speed and low distortion microscopy: on-field measurements and future evolution", Proc. SPIE 12274, Emerging Imaging and Sensing Technologies for Security and Defence VII, 122740D (7 December 2022); https://doi.org/10.1117/12.2636024
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KEYWORDS
Distortion
Microchannel plates
Microscopy
Electronics
Sensors
Avalanche photodiodes
Luminescence
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