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17 March 2023 FRET in small animal optical imaging: Intensity versus lifetime-based approaches
Jason T. Smith, Nattawut Sinsuebphon, Xavier Michalet, Xavier Intes, Margarida Barroso
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Proceedings Volume 12357, Visualizing and Quantifying Drug Distribution in Tissue VII; 1235707 (2023) https://doi.org/10.1117/12.2650586
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
Förster Resonance Energy Transfer (FRET) is a unique biophysical phenomenon that allows for energy transfer to occur between two light-sensitive molecules, e.g., a donor and an acceptor fluorophore. FRET has been leveraged in numerous biomedical applications to monitor molecular interactions at the nanoscale. Previously, we have developed a FRET-based nanometer-range proximity assay (2–10 nm) that measures receptor-ligand protein complexes during internalization and subsequent endocytic trafficking steps in vitro and in vivo. Recently, there has been great interest to perform FRET imaging in vivo, especially in the context of quantifying drug delivery in vivo.
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Jason T. Smith, Nattawut Sinsuebphon, Xavier Michalet, Xavier Intes, and Margarida Barroso "FRET in small animal optical imaging: Intensity versus lifetime-based approaches", Proc. SPIE 12357, Visualizing and Quantifying Drug Distribution in Tissue VII, 1235707 (17 March 2023); https://doi.org/10.1117/12.2650586
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KEYWORDS
Fluorescence resonance energy transfer
In vivo imaging
Imaging systems
Optical imaging
Calibration
Luminescence
Receptors
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