Comparative study of the influence of imaging resolution on polarization properties derived from Mueller matrix

Yuxin Zhang; Conghui Shao; Honghui He; Hui Ma

doi:10.1117/12.2648648

15 March 2023 Comparative study of the influence of imaging resolution on polarization properties derived from Mueller matrix

Yuxin Zhang, Conghui Shao, Honghui He, Hui Ma

Author Affiliations +

Proceedings Volume 12382, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023; 123820A (2023) https://doi.org/10.1117/12.2648648
Event: SPIE BiOS, 2023, San Francisco, California, United States

Conference Poster

Abstract

For pathological diagnosis using optical microscopes, imaging speed and resolution are two important considerations. Usually, the improvement of imaging resolution will reduce the field of view and greatly extend the imaging time. Recently, studies have shown that some polarization information obtained from Mueller matrix connecting with structural features can be preserved under relatively low imaging resolution. Here, to systematically investigate the influence of imaging resolution on polarization properties derived from Mueller matrix, we first perform transmission Mueller matrix microscopic imaging on the unstained rat dorsal skin tissue samples, which have rich fibrous structures. Then, we carry out quantitative analysis using the statistics and the image texture feature parameters to compare the Mueller matrix polarimetric parameters images at different resolutions. The results show that, compared with the traditional non-polarized microscopic images, the Mueller matrix polarimetric parameters, which can characterize the fiber density information of the sample, are less sensitive to the imaging resolution, while other polarimetric parameters derived from the Mueller matrix related to the particle sizes still need high resolution to provide accurate structural information. This study demonstrates that it is possible to consider both imaging resolution and speed when using Mueller matrix polarimetry for tissue detection, and proposes relevant criteria to meet the above requirements, which is of great significance for application scenarios that need accurate and highspeed optical measurement.

Citation Download Citation

Yuxin Zhang, Conghui Shao, Honghui He, and Hui Ma "Comparative study of the influence of imaging resolution on polarization properties derived from Mueller matrix", Proc. SPIE 12382, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023, 123820A (15 March 2023); https://doi.org/10.1117/12.2648648

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