Compressive time-resolved multispectral fluorescence microscopy with single-pixel camera

Alberto Ghezzi; Andrea Farina; Armin J. M. Lenz; Fernando Soldevila; Enrique Tajahuerce; Andrea Bassi; Gianluca Valentini; Cosimo D'Andrea

doi:10.1117/12.2648193

16 May 2023 Compressive time-resolved multispectral fluorescence microscopy with single-pixel camera

Alberto Ghezzi, Andrea Farina, Armin J. M. Lenz, Fernando Soldevila, Enrique Tajahuerce, Andrea Bassi, Gianluca Valentini, Cosimo D'Andrea

Author Affiliations +

Proceedings Volume 12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; 1238502 (2023) https://doi.org/10.1117/12.2648193
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Time-resolved multispectral fluorescence microscopy provides a 4D hypercube dataset with high specificity for cellular examination. However, this is generally obtained by significantly increasing the measurement time, which is quite limiting for in vivo measurement or with photosensitive sample. It is possible to reduce the measurement effort with a novel microscopy framework exploiting compressive sensing based on single-pixel camera. In this work, we present a compressive sensing system and validate it with a cellular sample. Data fusion with a high-resolution camera image allows us to tackle the well-known problem of low-resolution in single-pixel imaging.

Conference Presentation

Citation Download Citation

Alberto Ghezzi, Andrea Farina, Armin J. M. Lenz, Fernando Soldevila, Enrique Tajahuerce, Andrea Bassi, Gianluca Valentini, and Cosimo D'Andrea "Compressive time-resolved multispectral fluorescence microscopy with single-pixel camera", Proc. SPIE 12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, 1238502 (16 May 2023); https://doi.org/10.1117/12.2648193

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