Overview of the automatic reconstruction method for quantitative phase imaging using a digital holographic microscope operating in non-telecentric regime

Brian Bogue-Jimenez; C. Trujillo; A. Doblas

doi:10.1117/12.2651944

16 March 2023 Overview of the automatic reconstruction method for quantitative phase imaging using a digital holographic microscope operating in non-telecentric regime

Brian Bogue-Jimenez, C. Trujillo, A. Doblas

Author Affiliations +

Proceedings Volume 12389, Quantitative Phase Imaging IX; 123890B (2023) https://doi.org/10.1117/12.2651944
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Digital holographic microscopy (DHM), which provides quantitative phase imaging (QPI), has been widely applied in material and biological applications. The performance of DHM technologies relies heavily on computational reconstruction methods to provide accurate phase measurements. For example, non-telecentric DHM systems should compensate for the spherical wavefront associated with a non-telecentric configuration. The size of the ±1 diffraction orders in the hologram spectrum depends inversely on the radius of the curvature of the spherical wavefront introduced by the non-telecentric DHM system. Therefore, one can estimate the radius of curvature of the spherical wavefront by analyzing the hologram spectrum. Here, we outline the steps for the automatic reconstruction of phase images without distortions and with minimum user input from a hologram recorded in a non-telecentric DHM system. The proposed reconstruction approach can be divided into six main steps. The first step automatically selects the +1 diffraction order in the hologram spectrum. Secondly, the spherical wavefront parameters and the interference angle are estimated by analyzing the size and position of the selected +1 order. The third and fourth steps are the spatial filtering of the +1 order and the compensation of the interference angle, respectively. The next step involves the estimation of the center of the spherical wavefront. Finally, there is a fine-tuning step to optimize the estimated parameters and provide a phase image with minimum phase distortions. We have identified the relevant metrics in each step, compared multiple approaches, and selected the one with the higher performance for all our experimental holograms.

Conference Presentation

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Brian Bogue-Jimenez, C. Trujillo, and A. Doblas "Overview of the automatic reconstruction method for quantitative phase imaging using a digital holographic microscope operating in non-telecentric regime", Proc. SPIE 12389, Quantitative Phase Imaging IX, 123890B (16 March 2023); https://doi.org/10.1117/12.2651944

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