Rapid identification of antimicrobial drug resistance strains of E-coli using SERS nanowire chip

Sathi Das; Kanchan Saxena; Abdolrahman Khezri; Vishesh  Dubey; Azeem Ahmad; Jean-Claude Tinguely; Dalip Singh Mehta; Rafi Ahmad; Balpreet Singh Ahluwalia

doi:10.1117/12.2650578

16 March 2023 Rapid identification of antimicrobial drug resistance strains of E-coli using SERS nanowire chip

Sathi Das, Kanchan Saxena, Abdolrahman Khezri, Vishesh Dubey, Azeem Ahmad, Jean-Claude Tinguely, Dalip Singh Mehta, Rafi Ahmad, Balpreet Singh Ahluwalia

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Proceedings Volume 12396, Plasmonics in Biology and Medicine XX; 123960C (2023) https://doi.org/10.1117/12.2650578
Event: SPIE BiOS, 2023, San Francisco, California, United States

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Abstract

We have investigated the enhanced Raman spectra of AMR bacteria strains of E. coli using silver coated silicon nanowires SERS assay. Three different E. coli strains, E. coli CCUG17620, NCTC 13441, and A239, were detected using two different excitation laser wavelengths. We found stable and enhanced SERS spectrum using 785 nm laser as opposed to 532 nm. Future development of SERS-chip could offer a reliable platform for direct identification of the pathogen in bio-fluid samples at strains level.

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Sathi Das, Kanchan Saxena, Abdolrahman Khezri, Vishesh Dubey, Azeem Ahmad, Jean-Claude Tinguely, Dalip Singh Mehta, Rafi Ahmad, and Balpreet Singh Ahluwalia "Rapid identification of antimicrobial drug resistance strains of E-coli using SERS nanowire chip", Proc. SPIE 12396, Plasmonics in Biology and Medicine XX, 123960C (16 March 2023); https://doi.org/10.1117/12.2650578

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