Coregistration of multiphoton fluorescence with nanoscopic resolution atomic force microscopy

Adam Baker; Zhi Gao; Thomas Borg; Qi Wang; Tong Ye; Arman Kilic; Reece Fratus; Lucas Schmidt

doi:10.1117/12.2650709

15 March 2023 Coregistration of multiphoton fluorescence with nanoscopic resolution atomic force microscopy

Adam Baker, Zhi Gao, Thomas Borg, Qi Wang, Tong Ye, Arman Kilic, Reece Fratus, Lucas Schmidt

Proceedings Volume 12430, Quantum Sensing and Nano Electronics and Photonics XIX; 1243011 (2023) https://doi.org/10.1117/12.2650709
Event: SPIE OPTO, 2023, San Francisco, California, United States

Abstract

Maintaining the heart's health is one of the largest challenges in medicine due to the proclivity of life-threatening cardiovascular diseases, such as myocardial infarctions. When the heart experiences an infarction, a scar begins forming within an hour of the event, which will continue to grow and weaken the heart’s ability to contract. The myocardium after an infarction will increase in stiffness as the tissue becomes fibrotic; the influx of collagen dampens the flexion of the ventricles and reduces the cardiac output. The nature of the tissue stiffness is vital to understand not only at the tissue level but also at the mesoscopic domain. It is necessary to specify how the primary structural tissue components at the subcellular level contribute to the mechanical behavior of the muscle. To investigate this, we produced a procedure for mapping the mechanical nature of fresh myocardium: using atomic force microscopy to measure the mechanical properties of each structural component imaging determined by our second harmonic generation (SHG) microscopy. To coregistered AFM and SHG image, which has not been accomplished previously, we developed a convenient means of marking PDMS to be visible in SHG at 830 nm. Our research draws the line between the macroscopic mechanical behavior of the tissue to the nanoscopic structures.

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Adam Baker, Zhi Gao, Thomas Borg, Qi Wang, Tong Ye, Arman Kilic, Reece Fratus, and Lucas Schmidt "Coregistration of multiphoton fluorescence with nanoscopic resolution atomic force microscopy", Proc. SPIE 12430, Quantum Sensing and Nano Electronics and Photonics XIX, 1243011 (15 March 2023); https://doi.org/10.1117/12.2650709

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KEYWORDS

Atomic force microscopy

Tissues

Polydimethylsiloxane

Natural surfaces

Biological samples

Myocardium

Image registration

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