Hetero-substituted derivative of PtPFPP for intracellular O2 sensing of mammalian cells

Chiara Zanetti; Eimear Larkin; Anita R. Maguire; Dmitri B. Papkovsky

doi:10.1117/12.2669180

31 May 2023 Hetero-substituted derivative of PtPFPP for intracellular O₂ sensing of mammalian cells

Chiara Zanetti, Eimear Larkin, Anita R. Maguire, Dmitri B. Papkovsky

Proceedings Volume 12572, Optical Sensors 2023; 125721R (2023) https://doi.org/10.1117/12.2669180
Event: SPIE Optics + Optoelectronics, 2023, Prague, Czech Republic

Conference Poster

Abstract

Intracellular Optical Oxygen sensing is a convenient approach for monitoring and imaging molecular oxygen (O2) in biological samples, however existing intracellular O2-sensing probes still have some limitations. This study describes one new phosphorescent hetero-substituted derivative of Pt(II)-tetrakis(pentafluorophenyl)porphyrin (PtPFPP) obtained via two-step thiol click-chemistry. Particularly, thio-glucose (Glc) and thio-methyl-polyethylene-glycol (mPEG) moieties were covalently attached to the phosphorescent dye, producing the trans-di-glucosylated-di-PEGylated derivative PtGlc2PEG2 (trans). In a previous publication, we demonstrated the ability of these short PEG oligomers to drastically reduce the ability of the tetra- or tri-PEGylated conjugates to translocate across the cellular membrane. However, in this study, we show the capability of the trans-di-PEGylated-di-glucosylated conformation to allow intracellular staining and O2 sensing (IcO2) in murine embryonic fibroblasts (MEFs) mammalian cells.

Citation Download Citation

Chiara Zanetti, Eimear Larkin, Anita R. Maguire, and Dmitri B. Papkovsky "Hetero-substituted derivative of PtPFPP for intracellular O₂ sensing of mammalian cells", Proc. SPIE 12572, Optical Sensors 2023, 125721R (31 May 2023); https://doi.org/10.1117/12.2669180

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