Light sheet fluorescence microscopy in NIR II for mouse lung imaging

Chun-Chun Chang; S-Ja Tseng; Kuang-Yuh Huang; Yuan Luo

doi:10.1117/12.3007930

20 September 2023 Light sheet fluorescence microscopy in NIR II for mouse lung imaging

Chun-Chun Chang, S-Ja Tseng, Kuang-Yuh Huang, Yuan Luo

Proceedings Volume 12608, Biomedical Imaging and Sensing Conference; 1260816 (2023) https://doi.org/10.1117/12.3007930
Event: Optics and Photonics International Congress, 2023, Yokohama, Japan

Abstract

Light sheet fluorescence microscopy (LSFM) is one of the leading technique for high resolution imaging. To image mouse lungs, optical sectioning and high-speed image acquisition are required. In addition, light scattering, reflection, and attenuation limit the application of light sheet microscopy in the visible region to imaging small and transparent animals and organisms. In this work, we present a light sheet microscope that works in the NIR-II wavelength range and demonstrate deep mouse lung imaging. We utilized light with excitation at 532 nm and detection in the NIR-II range of approximately 1450 nm. Our method is expected to find important applications for deep tissue imaging.

Citation Download Citation

Chun-Chun Chang, S-Ja Tseng, Kuang-Yuh Huang, and Yuan Luo "Light sheet fluorescence microscopy in NIR II for mouse lung imaging", Proc. SPIE 12608, Biomedical Imaging and Sensing Conference, 1260816 (20 September 2023); https://doi.org/10.1117/12.3007930

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