Three-dimensional (3D) bioprinting approaches that enable large-scale constructs and high-resolution simultaneously are very attractive to tissue engineering applications and health industries. However, both characteristics hardly meet at reasonable printing times in current 3D bioprinting technologies, affecting the introduction of 3D scaffolds in medical applications. To overcome this limitation, we recently introduced a Vat Photopolymerization (VP)-based bioprinting method named Light Sheet Stereolithography (LS-SLA) and demonstrated the fabrication of centimeter-scale scaffolds with micrometer-scale features (⪆; 13 μm) by using off-the-shelf optical compounds. The high performance in LS-SLA results from using a rectangular uniform beam instead of a rotational symmetric laser beam, which generates light sheets with large length-to-width aspect ratios on the vat film. Beam shaping optics are components used to perform the beam transformation, and guarantee the accuracy, uniformity, and size of the 3D constructs. This work proposes freeform optics to perform the laser beam shaping in the LS-SLA device and describes the progress of our investigations from design to proof-of-concept-demonstrating. The results show that rectangular beams are readily produced by freeform optics resulting in compact and energy efficient systems, and that further considerations on the real laser output are necessary to deliver high beam uniformities. Tackling the design challenges of this work leads to energy efficient and high accuracy LS-SLA systems.
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