Comparison of nonlinear properties of monomer and dimer of bacterial phytochrome from Deinococcus radiodurans

Diana I. Galiakhmetova; Aleksandr S. Koviarov; Viktor V. Dremin; Andrei A. Gorodetsky; Marios Maimaris; Dmitrii A. Stoliarov; Mikhail Baloban; Vladislav V. Verkhusha; Sergei G. Sokolovski; Edik U. Rafailov

doi:10.1117/12.2666486

11 August 2023 Comparison of nonlinear properties of monomer and dimer of bacterial phytochrome from Deinococcus radiodurans

Diana I. Galiakhmetova, Aleksandr S. Koviarov, Viktor V. Dremin, Andrei A. Gorodetsky, Marios Maimaris, Dmitrii A. Stoliarov, Mikhail Baloban, Vladislav V. Verkhusha, Sergei G. Sokolovski, Edik U. Rafailov

Author Affiliations +

Proceedings Volume 12627, Translational Biophotonics: Diagnostics and Therapeutics III; 126272T (2023) https://doi.org/10.1117/12.2666486
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

Current medicine might be greatly enhanced by the ability to in vivo control and monitor neurons using opsins/phytochromes expressed in neural cells. The fundamental challenge with non-invasive neural cell activity regulation is a high absorption of visible light into biological tissues. This drawback could be mitigated by the photoconversion of phytochromes in spectral ranges with higher tissue transparency. In this study, we first demonstrated two-photon Pr→Pfr conversion of monomeric phytochrome at 1.2 μm wavelength. We did a comparison of linear and nonlinear conversion of truncated DrBphP bacterial phytochromes. This work provides a structured understanding of the optical properties of the dimer and monomer of phytochrome as well as their potential for use in optogenetics.

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Diana I. Galiakhmetova, Aleksandr S. Koviarov, Viktor V. Dremin, Andrei A. Gorodetsky, Marios Maimaris, Dmitrii A. Stoliarov, Mikhail Baloban, Vladislav V. Verkhusha, Sergei G. Sokolovski, and Edik U. Rafailov "Comparison of nonlinear properties of monomer and dimer of bacterial phytochrome from Deinococcus radiodurans", Proc. SPIE 12627, Translational Biophotonics: Diagnostics and Therapeutics III, 126272T (11 August 2023); https://doi.org/10.1117/12.2666486

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