Three-dimensional NIR-II tumor blood vessels and bone imaging using rotational stereo vision in small animals

Shih-Po Su; Huihua Kenny Chiang

doi:10.1117/12.2669899

9 August 2023 Three-dimensional NIR-II tumor blood vessels and bone imaging using rotational stereo vision in small animals

Shih-Po Su, Huihua Kenny Chiang

Author Affiliations +

Proceedings Volume 12628, Diffuse Optical Spectroscopy and Imaging IX; 1262819 (2023) https://doi.org/10.1117/12.2669899
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

Optical imaging in the second near-infrared (NIR-II, 1000–1700 nm) is suitable for visualizing tumor vascular imaging and targeting bone structures in small animals. In this study, we developed a novel three-dimensional (3D) NIR-II fluorescence rotational stereo imaging system for conducting 3D tumor vascular imaging and bone imaging in mice with only one NIR-II camera and a rotational stereo vision technique. Our system utilizes a 3D blood vessel reconstruction algorithm to present high-resolution 3D blood vessel maps and skeletal systems through two camera views. This technique allows for the visualization of bone structure and the identification of metabolic diseases such as osteoporosis. We validated the system with custom-made 3D printing phantoms and 4T1 tumor-bearing mice, demonstrating the ability to accurately recover the tumor blood vessels and bones with an imaging depth of 5 mm, an image resolution of 0.15 mm, and a depth resolution of 0.35 mm. This pioneering development provides an effective tool for non-invasive real-time NIR-II fluorescence imaging and is instrumental in understanding the effects of cancer treatments on tumor vessels and bone structure in small animals.

Citation Download Citation

Shih-Po Su and Huihua Kenny Chiang "Three-dimensional NIR-II tumor blood vessels and bone imaging using rotational stereo vision in small animals", Proc. SPIE 12628, Diffuse Optical Spectroscopy and Imaging IX, 1262819 (9 August 2023); https://doi.org/10.1117/12.2669899

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