Human-derived tumor-spheroid-based anti-cancer drugs testing using dynamic optical coherence tomography

I. Abd El-Sadek; R. Morishita; T. Mori; S. Makita; P. Mukherjee; S. Matsusaka; Y. Yasuno

doi:10.1117/12.2670856

11 August 2023 Human-derived tumor-spheroid-based anti-cancer drugs testing using dynamic optical coherence tomography

I. Abd El-Sadek, R. Morishita, T. Mori, S. Makita, P. Mukherjee, S. Matsusaka, Y. Yasuno

Proceedings Volume 12632, Optical Coherence Imaging Techniques and Imaging in Scattering Media V; 126321A (2023) https://doi.org/10.1117/12.2670856
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

We demonstrate label-free dynamic optical coherence tomography-based evaluation of tumor spheroids' response to multiple anti-cancer drugs based on different mechanisms. The study involved dynamic imaging of human breast cancer (MCF-7) spheroids treated with tamoxifen citrate (TAM), Paclitaxel (PTX), and doxorubicin (DOX). In addition, fluorescence (FLUO) imaging was performed as a reference. The D-OCT imaging is performed using a custom-built OCT device with a repeated raster scan protocol. Two algorithms, including Logarithmic Intensity Variance (LIV) and late OCT Correlation Decay Speed (OCDSl) were used to visualize the tissue dynamics. The D-OCT visualized the drug type-dependent morphological and tissue-activity response patterns of the tumor spheroid. The different response patterns are clearly explained by the mechanisms of the drugs. The presented results suggest that D-OCT can be a useful tool for anti-cancer drug testing.

Citation Download Citation

I. Abd El-Sadek, R. Morishita, T. Mori, S. Makita, P. Mukherjee, S. Matsusaka, and Y. Yasuno "Human-derived tumor-spheroid-based anti-cancer drugs testing using dynamic optical coherence tomography", Proc. SPIE 12632, Optical Coherence Imaging Techniques and Imaging in Scattering Media V, 126321A (11 August 2023); https://doi.org/10.1117/12.2670856

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