In situ molecular mapping of human testicular tissues by mid-infrared photothermal imaging

Jie Huang; PengCheng Fu; Zhicong Chen; Geng An; Delong Zhang; Hyeon Jeong Lee

doi:10.1117/12.2686651

27 November 2023 In situ molecular mapping of human testicular tissues by mid-infrared photothermal imaging

Jie Huang, PengCheng Fu, Zhicong Chen, Geng An, Delong Zhang, Hyeon Jeong Lee

Proceedings Volume 12766, Advanced Optical Imaging Technologies VI; 1276617 (2023) https://doi.org/10.1117/12.2686651
Event: SPIE/COS Photonics Asia, 2023, Beijing, China

Conference Poster

Abstract

Non-obstructive azoospermia (NOA) is a severe male infertility condition characterized by impaired or absent sperm production in the testes. The primary treatment for NOA is microsurgical testicular sperm extraction (micro-TESE), which relies on accurately identifying healthy seminiferous tubules. In addressing this clinical need, we propose the utilization of mid-infrared photothermal (MIP) microscopy to identify spectroscopic signatures associated with NOA. Our preliminary results revealed that NOA tissues exhibited distinctive lipid distribution and reduced lipid peak intensity compared to tissues with normal sperm production. Leveraging principal components analysis (PCA), we successfully extracted key infrared spectroscopic features. When combined with logistic regression (LR), this approach achieved an impressive prediction accuracy of 95.0% in classifying testicular tissues. These findings highlight the potential of MIP microscopy in facilitating sperm retrieval by distinguishing seminiferous tubules based on their molecular composition.

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Jie Huang, PengCheng Fu, Zhicong Chen, Geng An, Delong Zhang, and Hyeon Jeong Lee "In situ molecular mapping of human testicular tissues by mid-infrared photothermal imaging", Proc. SPIE 12766, Advanced Optical Imaging Technologies VI, 1276617 (27 November 2023); https://doi.org/10.1117/12.2686651

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