Super-diffraction limit focusing based on genetic algorithm design

Runze Li; Tong Peng; Chen Bai; Meiling Zhou; Baoli Yao

doi:10.1117/12.2686741

29 November 2023 Super-diffraction limit focusing based on genetic algorithm design

Runze Li, Tong Peng, Chen Bai, Meiling Zhou, Baoli Yao

Proceedings Volume 12768, Holography, Diffractive Optics, and Applications XIII; 1276822 (2023) https://doi.org/10.1117/12.2686741
Event: SPIE/COS Photonics Asia, 2023, Beijing, China

Conference Poster

Abstract

In point-scanning microscopy, such as confocal laser scanning microscopy (CLSM), the imaging resolution is limited by the size of the focal spot. Breaking through the diffraction limit by inserting a modulation phase mask in the objective pupil plane is a compact, high efficiency and adaptable scheme. Here, we propose a feedback phase design method based on genetic algorithm to control the 3D shape of the focal spot. The lateral and axial size can be jointly designed for various usages. Simulation and experimental results present the feasibility of this method. The axial size can be decreased by 50% while the lateral size is decreased by 15% simultaneously. This method of 3D super-diffraction limit focusing may potentially be used in CLSM for 3D super resolution imaging.

Citation Download Citation

Runze Li, Tong Peng, Chen Bai, Meiling Zhou, and Baoli Yao "Super-diffraction limit focusing based on genetic algorithm design", Proc. SPIE 12768, Holography, Diffractive Optics, and Applications XIII, 1276822 (29 November 2023); https://doi.org/10.1117/12.2686741

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