Pooria Iranian,1 Wannes W. Nevens,1 Thomas Lapauw,1 Thomas Van den Dries,1 Jan Coosemans,1 Sevada Sahakian,1 Théo Lepoutte,1 Valéry A. Jacobs,1 Hans Ingelberts,1 Maarten Kuijk1
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Fluorescence imaging is widely used in many different domains, one of which is fluorescence-guided surgery. The temporal behavior called fluorescence lifetime gives deeper insights, making it an additional tool. Current fluorescence-guided surgery imaging typically utilizes two different cameras for natural color and NIR fluorescence imaging, making the system complex and expensive. We propose an alternative approach of sequential RGB+NIR fluorescence lifetime imaging and overlaying the images on each other. Sequential RGB+NIR fluorescence imaging is achieved with a high-speed time-gated camera combined with an RGB LED ring illumination for natural color imaging and a picosecond NIR pulsed laser source for fluorescence lifetime imaging.
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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.
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Pooria Iranian, Wannes W. Nevens, Thomas Lapauw, Thomas Van den Dries, Jan Coosemans, Sevada Sahakian, Théo Lepoutte, Valéry A. Jacobs, Hans Ingelberts, Maarten Kuijk, "Novel sequential RGB+NIR fluorescence lifetime imaging with a single nanosecond time-gated CAPS camera," Proc. SPIE 12831, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XXII, 128310B (12 March 2024); https://doi.org/10.1117/12.3000970