The persistent emission that remains after excitation light irradiation is stopped enables high-contrast imaging without relying on surrounding autofluorescence. However, the luminance of common persistent emitting materials hardly increases even when the excitation light intensity is increased. Therefore, they have not been utilized for emission imaging in the nano-sized regions. Here we introduce high-resolution afterglow imaging using long-lived room temperature phosphorescence (RTP), which remains strongly emitted after excitation light irradiation is stopped. While looking at the correlation between the estimated value based on a unique dynamic calculation and the experimental value regarding RTP performance, we designed and synthesized molecules that are expected to improve the quantum yield of long-lived RTP. The RTP yield of the designed molecules has been greatly improved, allowing afterglow detection from small objects independent of autofluorescence. We introduce examples of afterglow imaging, where the resolution and contrast change depending on the excitation light intensity.
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