Creation of evanescent patterns at micron resolution by scanning an evanescent spot

Marc Grosjean; Alexei Grichine; Mylène Pezet; Olivier Destaing; Irène Wang

doi:10.1117/12.3017204

20 June 2024 Creation of evanescent patterns at micron resolution by scanning an evanescent spot

Marc Grosjean, Alexei Grichine, Mylène Pezet, Olivier Destaing, Irène Wang

Proceedings Volume 13006, Biomedical Spectroscopy, Microscopy, and Imaging III; 130060D (2024) https://doi.org/10.1117/12.3017204
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Total internal reflection fluorescence (TIRF) microscopy is a well-known technique allowing to confine the light close to the surface of a glass substrate. This axial confinement is based on the generation of evanescent waves. In TIRF microscopy however, there is no control of the light intensity in the transverse plane. Here, we propose a method to create evanescent patterns, which uses a fast-switching digital micro-mirror device to generate and scan an evanescent spot at multiple positions on the sample plane. In this way, patterns confined in the three dimensions of space can be produced in a fraction of second. This method would allow better spatial control in photo-activation or photo-conversion experiments in living cells, e.g. to target processes located at cell membranes.

Conference Presentation

Citation Download Citation

Marc Grosjean, Alexei Grichine, Mylène Pezet, Olivier Destaing, and Irène Wang "Creation of evanescent patterns at micron resolution by scanning an evanescent spot", Proc. SPIE 13006, Biomedical Spectroscopy, Microscopy, and Imaging III, 130060D (20 June 2024); https://doi.org/10.1117/12.3017204

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