Non-invasive near-infrared optogenetics: aspirational dream or approaching feasibility

Diana Galiakhmetova; Viktor Dremin; Aleksandr Koviarov; Dmitrii Stoliarov; Neville Ngum; Rheinallt Parri; Andrei Gorodetsky; Marios Maimaris; Daria Shcherbakova; Mikhail Baloban; Vladislav V. Verkhusha; Sergei Sokolovsky; Edik Rafailov

doi:10.1117/12.3016681

20 June 2024 Non-invasive near-infrared optogenetics: aspirational dream or approaching feasibility

Diana Galiakhmetova, Viktor Dremin, Aleksandr Koviarov, Dmitrii Stoliarov, Neville Ngum, Rheinallt Parri, Andrei Gorodetsky, Marios Maimaris, Daria Shcherbakova, Mikhail Baloban, Vladislav V. Verkhusha, Sergei Sokolovsky, Edik Rafailov

Author Affiliations +

Proceedings Volume 13007, Neurophotonics II; 1300703 (2024) https://doi.org/10.1117/12.3016681
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

This research focuses on the development of a non-invasive/minimally invasive optogenetic technique. The study delves into how visible (VIS) and near-infrared (NIR) light interacts with ex vivo mouse head tissues, highlighting the advantages of the NIR-II biological window for deeper tissue penetration and reduced light absorption and scattering. Our computer simulations and experimental results demonstrated that over 12% of initial light irradiation passes through 1 mm tissue (skin and skull), reaching the brain cortex, potentially enabling minimally invasive neural activation. Moreover, this work reveals the nonlinear optical properties of genetically engineered truncated monomeric and dimeric bacterial phytochromes, demonstrating their photoconversion efficiency of up to 73% in the NIR-II range and potential for optogenetics. This discovery opens new avenues in advanced neurostimulation and biomedical research by enhancing tissue penetration and minimizing invasiveness.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Diana Galiakhmetova, Viktor Dremin, Aleksandr Koviarov, Dmitrii Stoliarov, Neville Ngum, Rheinallt Parri, Andrei Gorodetsky, Marios Maimaris, Daria Shcherbakova, Mikhail Baloban, Vladislav V. Verkhusha, Sergei Sokolovsky, and Edik Rafailov "Non-invasive near-infrared optogenetics: aspirational dream or approaching feasibility", Proc. SPIE 13007, Neurophotonics II, 1300703 (20 June 2024); https://doi.org/10.1117/12.3016681

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