Surface plasmon resonance imaging signal amplification for the detection of micro-RNAs in the context of organ donation

Coline Beltrami; Julien Moreau; Laurence Convert; Jean-François Bryche; Paul G. Charette; Michael Canva

doi:10.1117/12.3016176

20 June 2024 Surface plasmon resonance imaging signal amplification for the detection of micro-RNAs in the context of organ donation

Coline Beltrami, Julien Moreau, Laurence Convert, Jean-François Bryche, Paul G. Charette, Michael Canva

Author Affiliations +

Proceedings Volume 13008, Biophotonics in Point-of-Care III; 130080A (2024) https://doi.org/10.1117/12.3016176
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Numerous medical conditions require extensive monitoring of diverse biomarkers, imposing a significant time and effort burden on healthcare professionals. This underscores the need for fast, reliable, and personalized point-of-care diagnostic tools. In the context of organ transplantation, the physiological status of a brain deceased organ donor requires comprehensive surveillance, accompanied by appropriate medical interventions to mitigate organ degradation. An effective strategy for monitoring this degradation involves tracking the progression and, ideally, the onset of the inflammatory response, the so-called ”cytokine storm”, by monitoring specific biomarkers: micro-RNAs. We propose two approaches based on a surface plasmon resonance imaging biosensor that combine various methodologies to amplify the hybridization signal of an oligonucleotide duplex (sandwich assay or bimaterial nanostructured biochip). In a proof-of-concept experiment involving a protein oligonucleotide complex in a sandwich assay, we successfully demonstrate signal amplification by one order of magnitude. Additionally, using the contribution of localized surface plasmons induced by nanostructuring the biochip, our simulations predict a further significant gain in amplification.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Coline Beltrami, Julien Moreau, Laurence Convert, Jean-François Bryche, Paul G. Charette, and Michael Canva "Surface plasmon resonance imaging signal amplification for the detection of micro-RNAs in the context of organ donation", Proc. SPIE 13008, Biophotonics in Point-of-Care III, 130080A (20 June 2024); https://doi.org/10.1117/12.3016176

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