Simultaneous auto-fluorescence and quantitative phase microscopy using transport of intensity equation: a label-free approach for cancer diagnosis

Himanshu Joshi; Pramila Thapa; Varun Surya; Krishna Agarwal; Dalip Singh Mehta

doi:10.1117/12.3021476

18 June 2024 Simultaneous auto-fluorescence and quantitative phase microscopy using transport of intensity equation: a label-free approach for cancer diagnosis

Himanshu Joshi, Pramila Thapa, Varun Surya, Krishna Agarwal, Dalip Singh Mehta

Author Affiliations +

Proceedings Volume 13010, Tissue Optics and Photonics III; 1301003 (2024) https://doi.org/10.1117/12.3021476
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

The present work shows design, development, and testing of the multimodal optical setup for simultaneous auto-fluorescence imaging, spectroscopy and quantitative phase microscopy (SAF-QPM) from same field of view (FOV). The SAF-QPM combines the capabilities of autofluorescence, offering molecular insights into metabolic activities, with quantitative phase imaging, providing nanometres-level sensitivity to cellular morphology and refractive index distribution. The autofluorescence serves as a biomarker for abnormal cellular changes associated with cancer development. Simultaneous incorporation of quantitative phase microscopy of the same tissue section probes the refractive index-dependent changes in the cellular morphology associated with cancer development. Non-interferometric QPI technique is used to retrieve the phase information of the sample, which overcomes the limitations of instability and coherent noise associated with interferometric QPI techniques. The incorporation of simultaneous AF and QPM from the same field of view enhances the accuracy and specificity of label-free cancer diagnosis.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Himanshu Joshi, Pramila Thapa, Varun Surya, Krishna Agarwal, and Dalip Singh Mehta "Simultaneous auto-fluorescence and quantitative phase microscopy using transport of intensity equation: a label-free approach for cancer diagnosis", Proc. SPIE 13010, Tissue Optics and Photonics III, 1301003 (18 June 2024); https://doi.org/10.1117/12.3021476

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available