Quantitative assessment of thin tumor depth and thickness in multi-layered silicone phantoms using low-resolution Raman spectroscopy

Subitcha Jayasankar; Sujatha Narayanan Unni

doi:10.1117/12.3016759

18 June 2024 Quantitative assessment of thin tumor depth and thickness in multi-layered silicone phantoms using low-resolution Raman spectroscopy

Subitcha Jayasankar, Sujatha Narayanan Unni

Author Affiliations +

Proceedings Volume 13010, Tissue Optics and Photonics III; 1301007 (2024) https://doi.org/10.1117/12.3016759
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Abstract

Perceiving the depth of flat and thin adenomas and the extent of invasion is vital in staging, diagnosis, treatment planning, and surgical precision. Raman spectroscopy and its spatially offset version have demonstrated excellent specificity in tissue classification and tumor margin analysis using label-free methods. In this work, we utilize fiber-optic Raman spectroscopy to simultaneously predict the depth and thickness of thin sub-surface tumors using tissue-like multi-layer phantoms. Silicone phantoms incorporating Hydroxyapatite distribution are used as a Raman scatterer to indicate malignant calcifications. The signal intensity for varied tumor depths and thicknesses is also numerically simulated and corroborated with experiments. The high-wavenumber Raman spectrum is captured using a fiber-optic low-resolution spectrometer with an excitation wavelength of 660 nm. The tumor's depth and thickness range from 0.5 mm to 2 mm in 0.5 mm increments. Partial Least Squares Regression (PLSR) analysis is carried out on the acquired dataset for predicting the tumor depth and thickness with a Root Mean Square Error (RMSE) of 0.268 mm (36.33%) and 0.120 mm (12.89%), respectively.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Subitcha Jayasankar and Sujatha Narayanan Unni "Quantitative assessment of thin tumor depth and thickness in multi-layered silicone phantoms using low-resolution Raman spectroscopy", Proc. SPIE 13010, Tissue Optics and Photonics III, 1301007 (18 June 2024); https://doi.org/10.1117/12.3016759

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available