FRET-based fiber-optic fluorescent biosensor for the detection of alpha-fetoprotein

Shiya Qi; Xuejin Li; Yuzhi Chen

doi:10.1117/12.3029172

6 May 2024 FRET-based fiber-optic fluorescent biosensor for the detection of alpha-fetoprotein

Shiya Qi, Xuejin Li, Yuzhi Chen

Proceedings Volume 13107, Fourth International Conference on Sensors and Information Technology (ICSI 2024); 131070U (2024) https://doi.org/10.1117/12.3029172
Event: Fourth International Conference on Sensors and Information Technology (ICSI 2024), 2024, Xiamen, China

Abstract

This article reports the development of a novel sensor based on fiber-optic fluorescence biosensing technology for detecting alpha-fetoprotein (AFP), a key biomarker for liver cancer screening. The sensor utilizes the fluorescence resonance energy transfer (FRET) effect and constructs a fluorescence immunoassay system on the surface of the optical fiber, using the sandwich method with dual antibodies. This approach leverages the immune response to quench the fluorescent microspheres with the Gold nanoparticles (AuNPs). By precisely quantifying the difference in light intensity before and after quenching, it establishes a direct correlation between the reduced light intensity and AFP concentration. The sensor boasts a broad detection range of 10–10,000 ng/mL and offers advantages such as compact size, affordability, and ease of operation, thus presenting a promising new method for AFP detection. Extensive experimental tests have been conducted to confirm the sensor's performance, demonstrating its high sensitivity and reliability under laboratory conditions. This research provides an innovative tool and method for liver cancer-related research and clinical applications, which is expected to promote further development in the field of liver cancer diagnosis.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Shiya Qi, Xuejin Li, and Yuzhi Chen "FRET-based fiber-optic fluorescent biosensor for the detection of alpha-fetoprotein", Proc. SPIE 13107, Fourth International Conference on Sensors and Information Technology (ICSI 2024), 131070U (6 May 2024); https://doi.org/10.1117/12.3029172

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