Probe-screened carbon nanotube field-effect transistor biosensor to enhance breast cancer-related gene assay

Pei Liu; Yiwei Liu; Bingjie Cai; Jiahao Li; Guojun Zhang

doi:10.1117/12.3036801

13 July 2024 Probe-screened carbon nanotube field-effect transistor biosensor to enhance breast cancer-related gene assay

Pei Liu, Yiwei Liu, Bingjie Cai, Jiahao Li, Guojun Zhang

Author Affiliations +

Proceedings Volume 13208, Third International Conference on Biomedical and Intelligent Systems (IC-BIS 2024); 1320806 (2024) https://doi.org/10.1117/12.3036801
Event: 3rd International Conference on Biomedical and Intelligent Systems (IC-BIS 2024), 2024, Nanchang, China

Abstract

BRCA1 gene testing is a trump card for liquid biopsy and early diagnosis in breast cancer, which has been limited, however, by the fact that the current mainstream methods such as gene sequencing are expensive, time-consuming and complex. In this study, a semiconductor carbon nanotube (CNT)-based field-effect transistor (FET) DNA sensor was constructed to detect BRCA1. The CNT-FET biosensors employed a floating gate (FG) structure and Y2O3 / HfO2 high κ bilayer gate dielectric. A key advance here is that the detection performance of three nucleic acid probes, DNA, phosphorodiamidate morpholino oligos (PMO) and peptide nucleic acid (PNA), was systematically investigated for the first time to define the optimal BRCA1 sensor configuration. The PNA-functionalized FG CNT-FET sensor stood out with an excellent sensitivity (LOD: 1.38 aM) and good specificity. What is more, the developed sensor also exhibited good anti-interference capability in serum BRCA1 testing, owing to the passivation of the bilayer dielectric. Importantly, the breast cancer patients and healthy individuals were successfully distinguished by our method, which is fully consistent with the gene sequencing results. This work represents a desirable analytical platform for the simple, sensitive, and accurate liquid biopsy of breast cancer. And we envision that the developed method will provide ideas for the construction of future DNA sensors and the selection of their recognition elements.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Pei Liu, Yiwei Liu, Bingjie Cai, Jiahao Li, and Guojun Zhang "Probe-screened carbon nanotube field-effect transistor biosensor to enhance breast cancer-related gene assay", Proc. SPIE 13208, Third International Conference on Biomedical and Intelligent Systems (IC-BIS 2024), 1320806 (13 July 2024); https://doi.org/10.1117/12.3036801

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