Vitamin A imaging in mammalian cells by hyperspectral stimulated Raman scattering microscopy

Xiangjie Huang; Wenkui Hou; Meixin Li; Wenyu Zhou; Delong Zhang; Wei Yan; Hyeon Jeong Lee

doi:10.1117/12.3034797

20 November 2024 Vitamin A imaging in mammalian cells by hyperspectral stimulated Raman scattering microscopy

Xiangjie Huang, Wenkui Hou, Meixin Li, Wenyu Zhou, Delong Zhang, Wei Yan, Hyeon Jeong Lee

Proceedings Volume 13242, Optics in Health Care and Biomedical Optics XIV; 132421E (2024) https://doi.org/10.1117/12.3034797
Event: SPIE/COS Photonics Asia, 2024, Nantong, Jiangsu, China

Abstract

Vitamin A, a fat-soluble vitamin essential for various biological processes, exists in multiple metabolic forms that are primarily converted in the liver. This complex and organ-specific metabolism depends on subcellular localization for proper conversion and function. Even within a single cell, the distribution of vitamin A is highly heterogeneous. Leveraging sub-micron spatial resolution and chemical selectivity, hyperspectral stimulated Raman scattering microscopy (hSRS) has been widely used to visualize metabolites in complex biological samples. Here, we employ (hSRS) imaging in fingerprint regions to visualize vitamin A distribution in mammalian cells.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Xiangjie Huang, Wenkui Hou, Meixin Li, Wenyu Zhou, Delong Zhang, Wei Yan, and Hyeon Jeong Lee "Vitamin A imaging in mammalian cells by hyperspectral stimulated Raman scattering microscopy", Proc. SPIE 13242, Optics in Health Care and Biomedical Optics XIV, 132421E (20 November 2024); https://doi.org/10.1117/12.3034797

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