Reusable fiber optic immunofluorosensor for rapid detection of pesticides

Nabil A. Anis; James J. Valdes; Roy G. Thompson; Darrell E. Menking; Rosie B. Wong; Mohyee E. Eldefrawi

doi:10.1117/12.144717

18 May 1993 Reusable fiber optic immunofluorosensor for rapid detection of pesticides

Nabil A. Anis, James J. Valdes, Roy G. Thompson, Darrell E. Menking, Rosie B. Wong, Mohyee E. Eldefrawi

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144717
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Quartz fibers coated with acetylcholinesterase (AChE) or antibody (Ab) are used as biosensors utilizing total reflectance fluorescence for the rapid detection of pesticides. The enzyme biosensor was constructed by immobilizing fluorescein isothiocyanate (FITC)-tagged eel electric organ AChE on quartz fibers. The fluorescent signal was generated by hydrolysis of acetylcholine (ACh) that is present in the perfusate. Organophosphate (OP) and carbamate anticholinesterase (AntiChE) insecticides inhibited AChE and reduced the fluorescent quenching resulting from AChE hydrolysis. A parathion biosensor was constructed by immobilizing casein-parathion on the quartz fibers, that bound rabbit antiparathion antibody. The optical signal was generated by perfusing the fibers with fluorescein-labeled goat antirabbit IgG. Free parathion inhibited the binding of antiparathion Abs and reduced the optical signal and provided the basis for detection of parathion. Another immunosensor developed detected the herbicide Pursuit^R by utilizing the reversible binding of a fluorescein-Pursuit derivative to antiPursuit Abs immobilized on the fiber. Unlabeled Pursuit competed effectively and displaced the bound fluorescent compound in a dose-dependent manner. The sensor discriminated effectively between Pursuit-like and structurally unrelated herbicides. The immunosensor offers the advantage of continuous monitoring, ease of operation, speed of detection, low cost, stability, specificity, matrix transparency, and reusability.

Citation Download Citation

Nabil A. Anis, James J. Valdes, Roy G. Thompson, Darrell E. Menking, Rosie B. Wong, and Mohyee E. Eldefrawi "Reusable fiber optic immunofluorosensor for rapid detection of pesticides", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144717

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