Fluorescence dynamics of upper layer of human skin under UV laser irradiation

Alexander B. Pravdin; Sergei R. Utz M.D.; A. A. Al'khov; Alexey N. Bashkatov

doi:10.1117/12.166830

15 January 1994 Fluorescence dynamics of upper layer of human skin under UV laser irradiation

Alexander B. Pravdin, Sergei R. Utz M.D., A. A. Al'khov, Alexey N. Bashkatov

Proceedings Volume 2081, Optical Biopsy; (1994) https://doi.org/10.1117/12.166830
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

In present work the temporal behavior of autofluorescence of upper (30 - 40 micrometers ) layer of human skin under continuous 337 nm laser exposure was investigated. The samples were prepared using epidermis stripping technique. Kinetic curves of autofluorescence decay at 420 nm, 440 nm, and 460 nm have been obtained. In double exponential approximation the parameters of autofluorescence decay have been evaluated. Under the assumption that different terms in double exponential approximation correspond to different individual fluorophores the contribution of fast bleaching, slow bleaching, and non-bleaching components to fluorescence spectrum of the sample at 420 nm, 440 nm, and 460 nm have been calculated.

Citation Download Citation

Alexander B. Pravdin, Sergei R. Utz M.D., A. A. Al'khov, and Alexey N. Bashkatov "Fluorescence dynamics of upper layer of human skin under UV laser irradiation", Proc. SPIE 2081, Optical Biopsy, (15 January 1994); https://doi.org/10.1117/12.166830

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